Box breathing is usually taught as equal counts of inhale, hold, exhale and hold, most often 4-4-4-4, with 4-3-3-3 as a variant some guides use. No trial found for this article compares the two counts. The broader evidence on slow breathing and stress is modestly positive but mixed, and the box pattern itself has been tested in only a few small studies.
What are 4-4-4-4 and 4-3-3-3?
Box breathing is a paced breathing pattern with four phases of equal or near-equal length: breathe in, hold, breathe out, hold again. The numbers are seconds per phase. In 4-4-4-4, each phase lasts four seconds, so one cycle takes 16 seconds, or 3.75 breaths a minute. In 4-3-3-3, the inhale is four seconds and the other three phases are three seconds, so a cycle takes 13 seconds, or about 4.6 breaths a minute. Those rates are our own arithmetic, not figures from a study. Both are slower than the pace of about six breaths a minute that much of the slow-breathing research centers on.3
How studies describe the pattern matters. The one randomized trial in this article that included a box breathing group, led by Balban, used equal phases and set the length of each phase from a CO2 tolerance test, anywhere from about 3 to 10 seconds per phase.1 By our arithmetic, that spans cycles of 12 to 40 seconds, or roughly five down to one and a half breaths a minute, so even within one trial the counts were not identical for everyone. The point is that researchers treat the count as adjustable to the person, not as a fixed recipe.
What did the trial with a box breathing group find?
Balban and colleagues randomized adults to one of four remote conditions, each five minutes a day for one month: cyclic sighing, box breathing, cyclic hyperventilation with retention, or mindfulness meditation. The box breathing group had 19 people and the meditation group 22.1 Box breathing did not differ significantly from meditation on positive mood, state anxiety or respiratory rate, and no significant HRV changes were observed in that group. The exhale-focused cyclic sighing condition was the one that improved mood and lowered respiratory rate more than meditation. If the exhale is the interesting part, see Why the exhale is the switch.
The authors list their own limits: a remote design with no way to check how closely people followed the protocol, only four weeks, small groups and self-reported surveys.1 Treat it as one small trial, not a verdict on box breathing.
What does the wider breathwork evidence say?
A meta-analysis of randomized trials of breathwork, by Fincham and colleagues, pooled 12 trials with 785 participants for self-reported stress. It found a small-to-medium reduction compared with control conditions (g = -0.35, 95% CI -0.55 to -0.14). Anxiety (20 trials, g = -0.32) and depressive symptoms (18 trials, g = -0.40) showed similar-sized effects.2 This covers many breathing methods, not box breathing specifically, and the authors call for higher-quality studies before firm conclusions. A small-to-medium effect also means the groups overlap a lot: on average the breathwork groups reported less stress, but many individuals in them did no better than the typical person in a control group.
A systematic review of slow breathing in healthy adults found 15 eligible studies out of 2,461 abstracts. Slow breathing, near six breaths a minute, went along with higher HRV and with lower anxiety and arousal and more reported relaxation. The techniques and participants differed too much for the studies to be combined statistically, and the studies generally lacked a justified sample size. It included only healthy adults aged 18 to 65, so it says nothing about people with health conditions.3
A larger systematic review and meta-analysis covered 223 studies and found that voluntary slow breathing increased vagally mediated HRV during the session, right after a single session and after multi-session programs (172 studies measured HRV during practice, 16 right after one session and 49 after multi-session programs). The authors describe slow breathing as a low-tech, low-cost technique with few adverse effects expected.4 HRV is best read as a trend in your own numbers, not a score to maximize; HRV, explained without the mysticism covers what it measures. The abstract does not report whether the ratio between phases or the breath holds changed the result.
Does the exact count matter?
Not in any way the current evidence can answer. No trial that turned up in the searches for this article compares 4-4-4-4 with 4-3-3-3, so a claim that one is better has no direct support here. The reviews above look at slow breathing as a group, and the abstracts we read do not break results out by phase ratio.
The one direct comparison involving a box pattern tested something different. After a 15-minute interval session on a stationary bike, 40 physically active university students did spontaneous breathing, 4-4-4-4 box breathing, or five seconds in and five seconds out (six breaths a minute). Final heart rate was higher with box breathing than with the six-breath pace (164.65 versus 154.77 bpm), and rated effort was higher, while recovery time did not differ significantly. HRV was not measured.5 This was a single session in young, fit people recovering from exercise, so it says little about winding down at a desk. It does suggest the holds can feel demanding, which is a reason to adjust the count to what is comfortable.
What these studies cannot tell you
Most outcomes are self-reported. People cannot be blind to which breathing pattern they are doing. Several trials are small, and results describe groups, so any one person may respond differently. Stress and anxiety here mean everyday experience measured with symptom scores, not diagnoses, and none of this evidence shows that a breathing practice treats or prevents any condition. The one trial with a box breathing group ran for four weeks, so it says nothing about practicing for months or years, and it cannot tell you whether any short-term change lasts. Reading the evidence this way keeps the claims no larger than the studies.
How to pick a pace for yourself
With no evidence separating the two counts, comfort is a reasonable guide. If four-second holds feel strained, a shorter hold such as 4-3-3-3, or no hold at all, is a legitimate choice. If you feel lightheaded, return to normal breathing. Pick a count you can repeat without effort, and treat what you notice (calmer, restless, no change) as information about you, not a pass or fail. A simple way to compare counts for yourself is to keep the practice short, change one thing at a time (for example the length of the holds), and note how you feel afterward, so the comparison comes from your own observations rather than from a claim the research cannot yet support.
Where iKima fits
iKima is a breathing and heart-rate app from Sentira Labs. This article is about the research, not about the app, and nothing here claims that any app or practice changes stress or health outcomes.
When to talk to a professional
This article is educational and is not medical advice. Breathing practices are self-help tools, not a treatment. If anxiety, low mood or physical symptoms are getting in the way of daily life, or if you have a heart or breathing condition, talk to a doctor or licensed mental health professional. If you are in crisis, contact local emergency services.
Questions people ask
Is 4-3-3-3 better than 4-4-4-4 for box breathing?
There is no trial in the evidence reviewed for this article that compares them, so neither can be called better. Both are slow, equal-phase patterns of fewer than five breaths a minute. Choose the count you can repeat comfortably.
Does box breathing reduce stress or anxiety?
The evidence is modest and mixed. A meta-analysis of breathwork trials found small-to-medium reductions in self-reported stress and anxiety, but the one trial with a box breathing group found no significant difference from mindfulness meditation. Studies measure symptom scores, not diagnoses, and breathing practices are not a treatment.
Does box breathing raise heart rate variability?
Slow breathing in general raises vagally mediated HRV, according to a meta-analysis of 223 studies. In the one trial that measured it in a box breathing group, no significant change was found. HRV is most useful as a trend in your own readings.
Do I have to hold my breath in box breathing?
The studies reviewed here do not test the holds separately, so their contribution is unknown. In one exercise-recovery trial, box breathing with holds produced a higher heart rate and higher effort ratings than a six-breath-a-minute pace without holds. If holds feel uncomfortable, shorten or skip them.
References
- Balban MY, Neri E, Kogon MM, Weed L, Nouriani B, Jo B, Holl G, Zeitzer JM, Spiegel D, Huberman AD. Brief structured respiration practices enhance mood and reduce physiological arousal. Cell Reports Medicine. 2023. doi:10.1016/j.xcrm.2022.100895 (Randomized controlled trial)
- Fincham GW, Strauss C, Montero-Marin J, Cavanagh K. Effect of breathwork on stress and mental health: a meta-analysis of randomised-controlled trials. Scientific Reports. 2023;13. doi:10.1038/s41598-022-27247-y (Meta-analysis)
- Zaccaro A, Piarulli A, Laurino M, Garbella E, Menicucci D, Neri B, Gemignani A. How breath-control can change your life: a systematic review on psycho-physiological correlates of slow breathing. Frontiers in Human Neuroscience. 2018. doi:10.3389/fnhum.2018.00353 (Systematic review)
- Laborde S, Allen MS, Borges U, Dosseville F, Hosang TJ, Iskra M, Mosley E, Salvotti C, Spolverato L, Zammit N, Javelle F. Effects of voluntary slow breathing on heart rate and heart rate variability: a systematic review and a meta-analysis. Neuroscience and Biobehavioral Reviews. 2022;138:104711. doi:10.1016/j.neubiorev.2022.104711 (Systematic review and meta-analysis)
- Kasap M, Aydin GR. Box breathing or six breaths per minute: which strategy improves athletes post-HIIT cardiovascular recovery? PLoS One. 2025. doi:10.1371/journal.pone.0336615 (Randomized crossover study)